← Back to All Case Studies CASE FILE #PET_URINE_SUBFLOOR_REMEDIATION
Residential Problem Cleaning Denver Metro, CO • February 2026 (2 Business Days)

Cat Urine Ammonia & Subfloor Saturation: Multi-Room Remediation

A rental property suffered 3 years of undetected feline urine saturation across 650 sq ft of carpeting, padding, and 3/4-inch OSB subflooring. Previous steam cleaning attempts had set protein bonds and exacerbated ammonia off-gassing.

Triage Similar Situation
INITIAL SITE AUDIT (PRE-CLEAN) CONTAMINATED
Surface Ammonia Vapor 32 ppm
OSB Subfloor Moisture 28.4%
395nm UV Fluorescence 84 sq ft active
Pre-ATP Swab Count 3,820 RLU
→
CLEARANCE PROTOCOL
POST-REMEDIATION (CLEARANCE) PASS / SANITARY
Surface Ammonia Vapor < 1 ppm (Non-Detectable)
OSB Subfloor Moisture 9.8% (Dry Standard)
395nm UV Fluorescence Zero Margins
Post-ATP Swab Count 22 RLU (Sanitary Pass)

Containment & Equipment Deployed

  • • Negative Air Scrubber (Dri-Eaz HEPA 500 CFM)
  • • Sub-Surface Extraction Hydro-Claw
  • • 395nm High-Intensity Ultraviolet Torch
  • • Tramex Moisture Encounter Non-Destructive Meter

Chemical Formulations & Dwell Protocol

  • • Bio-enzymatic bacterial spore concentrate (pH 7.8, 120-min damp dwell)
  • • Acidic neutralizer rinse (hydroxyacetic acid blend, pH 3.5)
  • • 2 coats Zinsser B-I-N White Pigmented Shellac Primer (odor barrier)

Itemized Financial Audit & Settlement

Total Project Invoiced: $1,840.00
Insurance Coverage Payout: $0.00 (Tenant Damage)
Client Net Out-of-Pocket / Deductible: $1,840.00 (Charged to Tenant Deposit)
Policy Settlement: Security Deposit Withholding + Itemized Invoicing

Primary Engineering Takeaway

"Never use heat or bleach on pet urine. High heat coagulates proteins, and bleach releases toxic chloramine gas when mixed with ammonia."

The Underlying Challenge

The property owner faced a complete lease renewal refusal due to intense eye-burning ammonia vapor. A previous carpet cleaning company applied high-temperature hot water extraction, which solubilized uric acid crystals and forced them 1.5 inches deep into the plywood subfloor joints.

The Scientific Remediation Protocol

Carpet and tack strips were removed under negative air containment. Subfloor was saturated with living enzyme culture (1.2 billion CFU/gal) and plastic-sheeting tented for 120 minutes to digest uric acid salts. Floor was dried with axial air movers to under 12% moisture, then sealed with two 3-mil coats of 100% dewaxed shellac.

Quantitative Verification & Clearance Result

Airborne ammonia dropped from 32 ppm to non-detectable. The landlord successfully passed tenant move-in inspection within 48 hours without replacing the subfloor.